ריצוף DNA – הבדלי גרסאות

תוכן שנמחק תוכן שנוסף
Matanyabot (שיחה | תרומות)
מ בוט החלפות: \1
שורה 27:
 
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|+ השוואת שיטות ריצוף מהדור החדש <ref name=quail2012>{{cite journal |last1=Quail |first1=Michael |last2=Smith |first2=Miriam E |last3=Coupland |first3=Paul |last4=Otto |first4=Thomas D |last5=Harris |first5=Simon R |last6=Connor |first6=Thomas R |last7=Bertoni |first7=Anna |last8=Swerdlow |first8=Harold P |last9=Gu |first9=Yong |display-authors=3 |title=A tale of three next generation sequencing platforms: comparison of Ion torrent, pacific biosciences and illumina MiSeq sequencers |journal=[[BMC Genomics]] |date=1 January 2012 |volume=13 |issue=1 |pages=341 |doi=10.1186/1471-2164-13-341 |pmid=22827831 |pmc=3431227 }}{{open access}}</ref><ref name=lin2012>{{cite journal |last1=Liu |first1=Lin |last2=Li |first2=Yinhu |last3=Li |first3=Siliang |last4=Hu |first4=Ni |last5=He |first5=Yimin |last6=Pong |first6=Ray |last7=Lin |first7=Danni |last8=Lu |first8=Lihua |last9=Law |first9=Maggie |display-authors=3 |title=Comparison of Next-Generation Sequencing Systems |journal=Journal of Biomedicine and Biotechnology |publisher=[[Hindawi Publishing Corporation]] |date=1 January 2012 |volume=2012 |pages=1&ndash;11 |doi=10.1155/2012/251364 }}{{open access}}</ref>
! שיטה !! Single-molecule real-time sequencing (Pacific Bio){{D}} !! Ion semiconductor (Ion Torrent sequencing) !!Pyrosequencing {{כ}}(454) !! ריצוף באמצעות סינתזה (אילומינה) !! ריצוף באמצעות ליגציה (ריצוף SOLiD) !! ריצוף סנגר
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שורה 38:
 
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| '''זמן הרצה''' ||30 דקות עד שעתיים <ref name=tran2012>{{cite journal |last1=Tran |first1=Ben |last2=Brown |first2=Andrew M.K. |last3=Bedard |first3=Philippe L. |last4=Winquist |first4=Eric |last5=Goss |first5=Glenwood D. |last6=Hotte |first6=Sebastien J. |last7=Welch |first7=Stephen A. |last8=Hirte |first8=Hal W. |last9=Zhang |first9=Tong |last10=Stein |first10=Lincoln D. |authorlink10=Lincoln Stein |last11=Ferretti |first11=Vincent |last12=Watt |first12=Stuart |last13=Jiao |first13=Wei |last14=Ng |first14=Karen |last15=Ghai |first15=Sangeet |last16=Shaw |first16=Patricia |last17=Petrocelli |first17=Teresa |last18=Hudson |first18=Thomas J. |authorlink18=Thomas J. Hudson |last19=Neel |first19=Benjamin G. |last20=Onetto |first20=Nicole |last21=Siu |first21=Lillian L. |last22=McPherson |first22=John D. |last23=Kamel-Reid |first23=Suzanne |last24=Dancey |first24=Janet E. |display-authors=19 |title=Feasibility of real time next generation sequencing of cancer genes linked to drug response: Results from a clinical trial |journal=[[Int. J. Cancer]] |date=1 January 2012 |pages=1547&ndash;1555 |doi=10.1002/ijc.27817 }}{{subscription required}}</ref>||שעתיים || 24 שעות || 1 עד 10 ימים, כתלות במרצף ובאורך הקריאות<ref name=vliet2010>{{cite journal |last=van Vliet |first=Arnoud H.M. |title=Next generation sequencing of microbial transcriptomes: challenges and opportunities |journal=[[FEMS Microbiology Letters]] |date=1 January 2010 |volume=302 |issue=1 |pages=1&ndash;7 |doi=10.1111/j.1574-6968.2009.01767.x }}{{open access}}</ref>||שבוע עד שבועיים || 20 דקות עד 3 שעות
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| '''למחיר למיליון בסיסים (בדולרים)''' ||$0.33-$1.00|| $1 || $10 || $0.05 עד $0.15 || $0.13 || $2400